S. Hossein Fatemi, MD, PhD
Timothy Griffin, PhD
University of Minnesota
Autism is a severe neurodevelopmental disorder with a rising prevalence of 18.5 per 1000 (1 in 54) in the United States. There have been few studies of the proteome of brains of individuals with autism.
Our long-range goal is to identify biomarkers for autism by combining subcellular fractionation and proteomics.
The objective of the current application is to determine the subcellular proteomes of individuals with autism vs. controls.
The central hypothesis is that proteomics will identify unique subcellular proteomes in subjects with autism vs. controls and that there will be differences related to age and brain region.
The rationale for the current proposal is that there is a dearth of brain subcellular proteomic studies related to autism. The project consists of the following specific aim:
Determine the proteome of the synaptic fraction in superior frontal cortex (BA9) and cerebellar vermis of subjects with idiopathic autism vs. healthy controls. Our work is innovative because these studies will provide important information about protein dysregulation in autism.